Preparation of corrected water

Important condition for successful autopathic treatment is the correct preparation of the dilution. In a situation where the person has chronic health problems, i.e. long-term problems that were treated but never cured through conventional medical methods, it is essential to proceed in the best possible and exact way according to proven and the most tested procedures. The autopathic bottle has already been implemented since six years and was proved to be effective in the treatment of thousands of cases of chronic health problems that could not be cured over a long period of time and were often causing much prolonged suffering (e.g. approximately 50 examples of cured cases described in my books about autopathy). The bottle has the advantage that it provides continuous flow-through dilution, which is very quick and exceptionally effective, it takes place in normal conditions and with the volume of water used we determine the exact potency to be made. We also have a guarantee that the dilution is not polluted by foreign biological information and that the material is free of contaminating leaching.

Exceptionally I have tried also other methods of infinitesimal dilution of saliva, particularly the Korsakoff method described in both of my books. It is a method of first aid, when no autopathic bottle is at hand. Low potencies made according to the Korsakoff method were usually effective in the first treatment, however, not in longer treatment. Korsakoff’s dilution has traditionally critics amongst homeopaths and many maintain that the potency stops at a level of 30 C irrespective of whether  we use two hundred or thousands steps. Treatment of chronic diseases takes however usually longer time, with repeated doses of autopathic preparation in higher potencies and here Korsakoff’s dilution in one vessel of random form fails. I have had several cases, especially at the beginnings of my autopathic practice, when we successfully used autopathic bottles, but then their manufacturer could not deliver the required quantity and I temporarily recommended going to the Korsakoff’s dilution. As far as I remember, I have always observed complications in healing, it came to a halt, new symptoms appeared, or already cured ones came back, etc. Only the further use of an autopathic bottle moved the case forward. In one such a case it was the treatment of eczema and migraine and in another juvenile arthritis. Both cases are now healed and without problems since several years, after having returned to the use of an autopathic bottle.

Once I had a similar case, which I described some years ago in the book get Well with Autopathy. The client came to me in the year 2004. Here a quote from the book:

„A man, 57 years old. High blood pressure, now up to 180/95, he’s had it for about a year, even though he’s taking medication against high blood pressure. The left chamber in his heart is dilated. He also takes medication for that. Sometimes he feels palpitations, sometimes his breathing is wheezy. The cholesterol in his blood is high – 6.3. His condition is deteriorating gradually. AP 3 litres, one dose. Check-up in five months. The breathing problems have passed, as have the palpitations, the cholesterol is down to 5.5 and the heart condition has improved, according to his doctor. The blood pressure is at a constant 135/75, or practically normal. He’s not sure if all these changes could have been caused by diluted saliva, but he knows of no other reason for these improvements, which came after applying the AP. He is booked for another check-up.“

Years of good condition followed, during which the same potency 240 C made in an autopathic bottle from six litres was repeated several times in a few situations of slightly increased blood pressure, short arrhythmia etc. The problems usually quickly passed after use of the autopathic preparation and did not return at all for a long time. In the check-up consultation in September 2007 he was completely without problems, with blood pressure 133/83 and with excellent resistance in stress situations in his profession and in mountain tourism. For the maintenance of the good condition I recommended repetition of autopathic dilution made with 6 l in regular intervals and made an appointment for a consultation in six month, which he did not keep.  Instead, he called me after one year that he has for some time the arrhythmias again, and this time bad ones. Somewhere he had read that it is possible to prepare potencies in the water bottle cap, into which he poured water. This he repeated in the agreed upon intervals, using the agreed upon quantity of water, but without the autopathic bottle until the previous pathology returned that was cured already years ago, and which did not react to his do-it-yourself prepared autopathic “potencies”. I sent him an autopathic bottle and recommended dilution with six litres. I asked him to come for a control to which he arrived totally healthy. The effect came within a week.

The basic finding from autopathic healing is that it is necessary to use finely differentiated potencies. A difference in 100 C can sometime be of major importance for full success. When we use various vessels, bottle caps etc., we do not know which potency develops (and whether it develops at all). On the other hand, potencies 30 C made with the Korsakoff method can be beneficial in acute situations and improve the condition temporarily. However, I am almost sure, that if the person does not switch over to an autopathic bottle, he or she will not stay with autopathy very long because the results achieved will not have long term effect. After my six years of experience I have not seen even one case of successful healing of long-term chronic diseases with potencies made in a haphazard way, while with autopathic bottle there are many.

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Introduction :

Culture media are available commercially as powder; they require only the addition of water. Nutrient medium is a general purpose preparation for culturing microorganisms which are not nutritionally fastidious.

The broth contains:

1.5 g/L “Lab-lemco” powder (a beef extract)
1.5 g/L yeast extract
5.0 g/L peptone (a nitrogen source)
5.0 g/L sodium chloride
15.0 g/L agar powder

The agar prepared has the same composition. The final pH of both media is 7.4.

Autoclaving

Autoclaving is a process that use moist heat and pressure so that all parts of the material to be sterilized reach 121 degree celcius for 15 minutes. An autoclave is, in essence, a large pressure cooker; a chamber which may be sealed off against surrounding air.

Materials for sterilization are placed in the chamber, the door is sealed, and pressurized steam is forced into the chamber. The incoming steam displaces cooler air through an exhaust valve; this valve closes when the cell cooler air has been vented.

Steam is continually forced into the chamber until the pressure reaches 103 kPa above atmospheric pressure; at sea level, this pushes the temperature in the chamber to 121 degree celcius. The high pressure prevents solutions from boiling over at this temperature. Larger volumes require longer than 15 minutes to heat up to 121 degree celcius throughout. After sterilization, the steam pressure is slowly decreased to atmospheric pressure. The sterilized objects can then be removed.

Objective:

To prepare sterile nutrient agar for culturing microorganisms.

Material and reagents:

Commercial nutrient agar,Balance,Distilled water,Scott bottles,Measuring cylinder
Beaker,Forcep,Universal bottles

Procedure

1. Appropriate amount of broth (with agar) powder is weighed into Scott bottles and dissolve with distilled water. They are mixed well.

2. The bottles are loosely recap and set aside for sterilization.

3. All the media are sterilized at 121 degree celcius for 15 minutes.

4. After autoclaving, the media is removed. The broth preparation is allowed to cool and the cap of each bottle is tightened.

Discussion:

There are several precaution steps we need to take when handling the experiment.

1) Balance

  • The appropriate amount of broth powder and agar powder is weighed using electronic analytical balance which has the precision of one hundredth of a gram, ±0.01 or one ten-thousandth of a gram, ±0.0001 g.
  • The proper receiver for the material must be selected. The receiver’s weight plus the weight to be measured must not exceed the maximum load for the balance. The size and shape of the receiver should permit it to fit into the space and on the balance pan without interfering with any operation. It is important that the receiver is clean and in dry condition. Common receivers are weighing bottles,weighing funnels,flasks,and weighing paper. The correct receiver depends upon the quantity and type of material (liquid,solid,or powder)to be weighed.
  • Make sure the surrounding of the pan and the pan of the balance is clean. Place the receiver on the center of the pan of the balance and close the balance door. Then, press the appropriate tare key on the balance to set the signal from the strain gauge to zero so that the weight of the receiver is no longer indicated. Carefully add the powdered material using a spatula until the desired amount is added. Handle with care to avoid spilling.
  • If solids are spilled, remove the receiver and sweep out all of the spilled material from the balance using a brush.The spilled material must be properly disposed.
  • To be effective the autoclave must reach and maintain a temperature of 121-123 degree celcius for at least 30 minutes. This is achieved by using saturated steam under at least 15 psi of pressure.

2) Autoclaving process

  • Check the drain screen at the bottom of the chamber before using the autoclave.
  • Clean out any debris for efficient heat transfer as steam must flush out of the autoclave chamber. If the drain screen is blocked with debris, a layer of air may form at the bottom of the autoclave and prevent proper operation.                                                                      
  • Make sure that the  water level is higher than the material in the autoclave                          
  • Make sure the water level should between range of low and high. If there are too low water level, water should be added in.                                                                                                                  
  • Make sure the cap of the Scott bottles must not too tight to prevent breakage off the Scott bottles.                      
  • Make sure the cap of the Scott bottles must not too loose to prevent the outflow of media inside the Scott bottles.      

                                                                                               We loosen the cap to allow the expansion of the bottle                                                               so that the bottle will not break.                                 

  • Autoclave doors must be firmly locked into place before running the autoclave.                                                                                                                                       
  • Do not stack or store combustible material next to an autoclave (cardboard, plastic, volatile or flammable liquids).  
  • Always use heat resistant gloves when removing materials after sterilization.       
  • Avoid touching the inner chamber surfaces after sterilization.

3) Agar

  • There are a few types of general nutrient agar plates.
  • Luria Bertani (LB) agar is a common nutrient agar for the general routine growth of bacteria and is not preferentially suited toward a particular microbe type.
  • Miller’s LB agar is a variety of LB containing different proportions of the same components.
  • Trypticase Soy agar (TSA) is another general purpose medium made with casein and soybean meal and is used as initial growth medium to observe bacterial morphology or increase bacterial growth for analysis or storage.
  • Phenylethyl alcohol agar (PEA) is selective for species of Staphylococcus and inhibits Gram-negative bacteria.
  • Brain Heart Infusion (BHI) agar is a general purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and moulds. The BHI agar derives its nutrients from the brain heart infusion, peptone and dextrose components. The peptones and infusion are sources of organic nitrogen, carbon, sulfur, vitamins and trace substances. Dextrose is the carbohydrate source that microorganisms utilize by fermentation action. The medium is buffered through the use of disodium phosphate.

Other precautions:

  • When preparing commercial media, we must read the label and instruction on the container before use.                                                                                                                                  
  • In the progress of experiment, use distilled water to clean all the apparatus.                                                                           
  • Measuring cylinder is used to measure the volume of distilled water required accurately.
  • Stir the mixture continuously to ensure that the nutrient powder dissolves completely.

Conclusion:

Different types of agar are needed for the cultivation of different types of microorganisms. Agar of the same composition with the commercial agar can be made by following the correct procedures. Preparation and sterilization of culture media should be done with great care to avoid contamination of unwanted microorganisms. We had learnt the preparation and sterilization of culture media via autoclaving process and the precaution steps that we need to take into consideration  when handling this experiment.

Reference:

http://www.studentsguide.in/animal-biotechnology/animal-cell-and-tissue-culture/preparation-and-sterilization-of-medium.html

http://en.wikipedia.org/wiki/Autoclave

http://www.csudh.edu/oliver/demos/bal-use/bal-use.htm

http://www.newdruginfo.com/pharmacopeia/usp28/v28230/usp28nf23s0_c1251.htm

http://www.ehow.com/info_8131230_types-agar-plates.html

http://www.bd.com/ds/technicalCenter/inserts/L007442(09)(201101).pdf

http://www.bionique.com/…/better-aseptic-technique.html – United States

georgelab.eng.uci.edu/resources/Aseptic%20Technique.pdf

ibg102labreports.wordpress.com

On this page you will find an explanation of a drinking water purification process. All process steps are numbered and the numbers correspond with the numbers in the schematic representation of the drinking water process found below. This is a summing up of the process steps:

a: Prefiltration

1) The uptake of water from surface waters or groundwater and storage in reservoirs. Aeration of groundwater and natural treatment of surface water usually take place in the reservoirs. Often softening and pH-adjustments already happen during these natural processes.

2) Rapid sand filtration or in some cases microfiltration in drum filters.

b: Addition of chemicals

3) pH adjustment through addition of calcium oxide and sodium hydroxide.

4) FeCl3 addition to induce flocculation for the removal of humic acids and suspended particulate matter, if necessary with the addition of an extra flocculation aid. Flocs are than settled and removed through lamellae separators. After that the flocs are concentrated in sludge and pumped to the exterior for safe removal of the particulates and sludge dewatering.

5) Softening in a reservoir, through natural aeration or with sodium hydroxide, on to 8,5 oD. This is not always necessary. For instance, in case natural filtration will be applied, softening takes place naturally.

c: Natural filtration

6) Drinking water preparation step that is specific for the Netherlands: Infiltration of the water in sand dunes for natural purification. This is not applied on all locations The water will enter the saturated zone where the groundwater is located and it will undergo further biological purification. As soon as it is needed for drinking water preparation, it will be extracted through drains.

d: Disinfection

7) Disinfection with sodium hypochlorite or ozone. Usually ozonation would be preferred, because ozone not only kills bacteria and viruses; it also improves taste and odour properties and breaks down micro pollutants. Ozone diffuses through the water as small bubbles and enters microrganisms cells by diffusion through cell walls. It destroys microrganisms either by disturbance of growth or by disturbance of respiratory functions and energy transfers of their cells. During these processes ozone is lost according to the reaction O3 -> O2 +(O).

e: Fine filtration

8) Slow sand (media) filtration for the removal of the residual turbidity and harmful bacteria. Sand filters are backwashed with water and air every day.

9) Active carbon filtration for further removal of matter affecting taste and odour and remaining micro pollutants. This takes place when water streams through a granular activated carbon layer in a filter. Backwash is required regularly due to silting up and reactivation of an active carbon filter should be done once a year.

f: Preservation and storage

10) Addition of 0.3 mg/L sodium hypochlorite to guarantee the preservation of the obtained quality. Not all companies chlorinate drinking water. The water will eventually be distributed to users through pipelines and distribution pumps.

11) Aeration for recovery oxygen supply of the water prior to storage. This is not always applied.

12) Remaining water can be stored in drinking water reservoirs.

In the following schematic representation of the drinking water preparation process dotted arrows represent the incoming chemicals and red arrows represent the outgoing flows.

Schematic representation of the drinking water preparation process

Water is not always infiltrated in sand dunes during treatment. Holland clearly illustrates this:
– In Rotterdam water is stored in reservoirs in the Biesbosch, where it undergoes natural treatment
– In Amsterdam the water was stored and naturally treated in sand dunes on to the year 2000, now it is stored in reservoirs
– In The Hague the water is still stored and naturally treated in sand dunes

For the answer to your questions on drinking water, check out our drinking water FAQ

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